采用反相乳液法制备了一种新型的自固化磷酸钙微球,其粒径范围为100～1000μm,中位径在388～605μm之间,内部有43.4%～66.7%的凝胶孔和微孔.通过正交试验获得了稳定制备微球的优化工艺参数：搅拌速度150r/min,明胶质量浓度8.0g/mL,油水相体积比10∶1,液固比2.0∶1.文中还研究了在此工艺条件下制备的自固化磷酸钙微球的流动性、力学性能、抗崩解性及细胞相容性.结果表明：所制备微球的流动性良好,自然堆积安息角为28.4°～31.6°;微球有良好的韧性,不易破碎,经过充分水化后,微球的抗压性能显著提高;微球在模拟生理条件下具有良好的抗崩解性和良好的细胞相容性.

The novel self-setting calcium phosphate microspheres were prepared by the employment of a reversed emulsion method. The diameter of the microspheres distributed from 100 μm to 1000 μm with a median diameter between 388~605μm, with a porosity between 43.4%~66.7%. The processing parameters for preparation of the microspheres were optimized to be: an agitating speed of 200r/min, a gelatin concentration of 8%w/v, an oil to water ratio of 5:1, and a liquid to powder ratio of 2.0. The angle of repose of the microspheres was measured to be 28.4°~31.6°, indicating good flowability. The compressive test showed that the microspheres were non-brittle, which is in favor of clinical applications. The compressive performance of the microspheres was significantly improved after full hydration at 37°C and 97% humidity. Anti-disintegration test revealed the microspheres was endurable in simulated physiological conditions and MTT test indicated the microspheres were biocompatible. The self-setting calcium phosphate microspheres were a potential candidate for bone defect filling at non-load-bearing sites and drug carrier.