对高效液相色谱的色谱条件进行优化，建立灵芝三萜类成分的指纹图谱分析方法，拟定赤芝、紫芝指纹图谱的共有模式，并将其初步应用于评价赤芝与紫芝的质量差异。优化后的流动相为乙腈-2％冰醋酸（梯度洗脱），检测波长为252nm，流速为1mL／min，柱温为35℃，14批赤芝、7批紫芝共标示出29个共有峰．鉴别了3个成分（灵芝酸A、C2，G），发现灵芝酸A的峰最强；赤芝与紫芝的共有峰相对比值较接近，但紫芝的单位生药峰面积只有赤芝的5％～10％左右，说明含灵芝制剂的质量评价应采用指纹图谱与含量测定相结合的方法．采用优化后的方法获得的灵芝三萜指纹图谱表明，赤芝三萜类成分较其它文献报道多，紫芝中三萜类成分能明显检出，说明该方法准确可靠，重复性好．

By optimizing the experimental conditions of high-performance liquid chromatography （ HPLC）, a method of fingerprint analysis of Glossy ganoderma was presented and a common mode of the fingerprints of Ganoderma lucidum and Ganoderma sinense was proposed, which were both initially used to estimate the quality difference. The HPLC conditions were optimized as： acetonitrile-2% acetic acid solution （gradient elution） as a mobile phase, a detection wavelength of 252 nm, a flow rate of 1 mL/min and a column temperature of 35 ℃. In the optimal conditions, 29 common peaks were separated from 14 batches of Ganoderma lucidum and 7 batches of Ganoderma sinense, and three compositions （ganoderic acids A, C2, G） were identified. The results indicate that the peak of ganoderic acid A is of the strongest intensity ; and that although the relative ratio of common peaks of Ganoderma lu- cidum is close to that of Ganoderma sinense, the peak area per unit medicine of Ganoderma sinense is only about 5% -10% of that of Ganoderma lucidum, which means that the quality evaluation of the products from Glossy ganoderma should combine both the HPLC fingerprint and the content determination. Moreover, according to the fingerprints of triterpenoids from Glossy ganoderma obtained by the optimized method, it is found that the Ganoderma lucidum contains much more triterpenoid compositions than what is reported in the literature, and that the triterpenoids in Ganoderma sinense can be easily detected. It is, thus, concluded that the proposed method is reliable, accurate and reproducible.