食品科学与技术

蛋清抗氧化肽增效剂的优化

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  • 1.吉林大学 军需科技学院, 吉林 长春 130062; 2.吉林大学 生物与农业工程学院, 吉林 长春 130025
林松毅(1970-),女,博士,副教授,主要从事功能食品研究与开发.

收稿日期: 2010-02-04

  修回日期: 2010-03-23

  网络出版日期: 2010-08-25

基金资助

国家“863”计划项目(2007AA10Z329);中央高校基本科研业务费科学前沿与交叉学科创新项目(200810029)

Optimization of Synergist of Antioxidant Peptide Derived from Egg White Protein

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  • 1. College of Quartermaster Technology, Jilin University, Changchun 130062, Jilin, China; 2. College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, Jilin, China
林松毅(1970-),女,博士,副教授,主要从事功能食品研究与开发.

Received date: 2010-02-04

  Revised date: 2010-03-23

  Online published: 2010-08-25

Supported by

国家“863”计划项目(2007AA10Z329);中央高校基本科研业务费科学前沿与交叉学科创新项目(200810029)

摘要

以DPPH(1,1-二苯基-2-苦肼基)清除率为评价指标,考察了NaCl、糖类、柠檬酸单独添加以及两两或三者合用对蛋清抗氧化肽活性的影响,并在此基础上运用响应面分析法,优化出增效剂的最佳组合.结果表明,NaCl、麦芽糖及柠檬酸均能显著提高蛋清抗氧化肽的活性,可作为蛋清抗氧化肽的抗氧化增效剂单剂,三者合用时增效效果最佳,最佳组合为NaCl1.4%、麦芽糖3.0%、柠檬酸0.24%,在此条件下蛋清抗氧化肽对DPPH的IC50值(DPPH清除率达到50%时蛋清抗氧化肽的质量浓度)为0.27mg/mL,增效比可达3.96.

本文引用格式

林松毅 郭洋 王莹 刘静波 郑淋 殷涌光 . 蛋清抗氧化肽增效剂的优化[J]. 华南理工大学学报(自然科学版), 2010 , 38(8) : 100 -104 . DOI: 10.3969/j.issn.1000-565X.2010.08.019

Abstract

This paper focuses on the effects of single or complex addition of NaCl, carbohydrates, citric acid on the antioxidant activity of the peptide derived from egg white protein, with the DPPH scavenging activity as the evaluation index. Moreover, the best combination of synergist is optimized via the response surface methodology. The results show that ( 1 ) NaCl, maltose and citric acid all signifieantly improve the antioxidant activity of the peptide derived from egg white protein, so that they can be used as an antioxidant synergist independently; (2) the triadic combination of NaCl, maltose and citric acid is of the best enhancement effect ; and (3) in the optimal combination ( i. e. , 1.4% NaCl, 3.0% maltose and 0. 24% citric acid), the synergist may result in a IC50 value on DPPH radicals of 0.27 mg/mL and a synergism value being up to 3.96.

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