收稿日期: 2023-03-03
网络出版日期: 2023-08-16
基金资助
宁夏回族自治区重点研发计划项目(2021BEG02027)
Inhibitory Effects and Molecular Mechanism of Wheat Antioxidant Peptides on Oxidative Stress Injury in Human Embryonic Kidney Cells
Received date: 2023-03-03
Online published: 2023-08-16
Supported by
the Key R&D Program of Ningxia Hui Autonomous Region(2021BEG02027)
通过2，2-偶氮二异丁基脒二盐酸盐（AAPH）诱导建立人胚肾细胞（HEK293）氧化应激损伤模型，评价5种小麦蛋白源肽Leu-Tyr（LY）、Pro-Tyr（PY）、Tyr-Gln（YQ）、Ala-Pro-Ser-Tyr（APSY）和Arg-Gly-Gly-Tyr（RGGY）的抗氧化活性，然后利用量子化学计算和分子对接技术预测5种小麦蛋白源肽与2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）结合的最优构型和结合作用，探讨小麦蛋白源肽发挥活性的分子机制。细胞试验结果表明：5种小麦蛋白源肽作用后，细胞死亡率显著下降到3.68%以下（P < 0.05），并且能显著减少AAPH诱导的活性氧自由基（ROS）生成（P < 0.05），使ROS含量趋于正常水平；5种小麦蛋白源肽均呈现出较好的总抗氧化能力和1，1-二苯基-2-三硝基苯肼（DPPH）自由基清除能力（P < 0.05），RGGY的总抗氧化能力最强，活性值为（1.46 ± 0.08）mmol/L Trolox，其次是APSY、YQ、PY和LY；YQ的DPPH自由基清除能力最强，清除率为61.34% ± 2.24%，其次是APSY、RGGY、PY和LY。分子对接结果表明，5种小麦蛋白源肽的CDOCKER交互能量（-CIE）评分分别为13.304 9、13.397 3、13.412 1、16.768 5、16.268 3，均可以有效地与ABTS相互作用，主要通过与ABTS分子之间形成较强的氢键和疏水作用力来发挥抗氧化活性。
刘文颖, 任杰, 吴晗硕, 等 . 小麦抗氧化肽对人胚肾细胞氧化应激损伤的抑制作用及分子机制[J]. 华南理工大学学报(自然科学版), 2024 , 52(4) : 33 -41 . DOI: 10.12141/j.issn.1000-565X.230082
The study firstly established oxidative stress injury model of human embryonic kidney cells (HEK293) by the induction of 2,2-azobis (2-methylpropionamidine) dihydrochloride (AAPH) to evaluate the antioxidant activity of five wheat protein-derived peptides Leu-Tyr (LY), Pro-Tyr (PY), Tyr-Gln (YQ), Ala-Pro-Ser-Tyr (APSY) and Arg-Gly-Gly-Tyr (RGGY). Then, it used quantum chemistry and molecular docking techniques to predict the optimal configuration and binding effect of five wheat protein-derived peptides combined with 2,2-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), and to explore the molecular mechanism of wheat protein-derived peptides. The results of cell test show that after the action of five wheat protein-derived peptides, the cell death rate significantly decreases to below 3.68% (P < 0.05), and the generation of reactive oxygen species (ROS) induced by AAPH was significantly reduced (P < 0.05), making the ROS content tend to normal levels. All five wheat protein-derived peptides show good total antioxidant capacity and free radical scavenging capacity of 1,1-Diphenyl-2-picrylhydrazyl (DPPH) (P < 0.05). RGGY shows the strongest total antioxidant capacity, with an activity value of (1.46 ± 0.08) mmol/L Trolox, followed by APSY, YQ, PY and LY. The DPPH free radical scavenging ability of YQ is the strongest, with a scavenging rate of 61.34% ± 2.24%, followed by APSY, RGGY, PY and LY. The results of molecular docking show that the CDOCKER interaction energy (-CIE) scores of the five wheat protein-derived peptides are 13.304 9, 13.397 3, 13.412 1, 16.768 5 and 16.268 3, respectively, which can effectively interact with ABTS, mainly through the formation of strong hydrogen bonds and hydrophobic forces between ABTS molecules to exert antioxidant activity.
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