为探讨酵母酶解物（YH）对H2O2引起的人脐静脉内皮细胞（HUVEC）氧化损伤的保护机制，研究了YH的抗氧化活性。结果显示：YH对1，1-二苯基-2-三硝基苯肼（DPPH）清除的半数效应浓度（EC50）为1.85mg/mL，具有良好的抗氧化活性；高剂量（500μg/mL）的YH可以极显著地减轻H2O2对HUVEC细胞增殖的抑制作用，极显著地减少细胞中丙二醛（MDA）的生成，并显著提升细胞内超氧化物歧化酶（SOD）酶活，从而改善由H2O2造成的细胞氧化损伤。转录组学测序结果显示，YH可显著上调SOD2和HMOX1基因转录水平。研究结果为YH改善内皮细胞氧化损伤提供基础数据，并为其在抗氧化功能食品及医药领域的应用提供了理论依据。

To investigate the protective mechanism of yeast hydrolysate （YH） on human umbilical vein endothelial cell injury induced by H2O2，antioxidant activity of YH was studied.The results show that the median effective concentration （EC50） of YH on DPPH scavenging is 1.85 mg/mL，showing good antioxidant activity.And YH of high-dose （500 μg/mL） can significantly alleviate the cell proliferation inhibition induced by H₂O₂，reduce malondialdehyde （MDA） production and increase the level of superoxide dismutase （SOD），thereby resisting cell oxidative damage of HUVEC caused by H₂O₂.Moreover，transcriptomics sequencing reveals that YH  can significantly up-regulate the expression of genes SOD2 and HMOX1.The research results not only provide basic data for YH to reduce endothelial cell oxidative damage，but also provide a theoretical basis for the application of YH in the fields of antioxidation functional foods and medicine.