为研究四种食源肽经口摄入后对光老化皮肤中氧化和炎症系统的影响，将 SPF 级 SD 大鼠均分为空白对照组、紫外辐照组和肽干预组． 4 种肽分别配制为0． 3、 0． 9、 1． 5g/L的含肽水溶液，作为唯一水源行低、中、高剂量经口自由摄入． 所有动物背部局部 区域均行脱毛处理，之后除正常对照组外，所有其他组的大鼠均连续紫外辐照至模型组呈 现典型光老化表观特征，然后取背部裸露部位皮肤制备组织匀浆液，并检测其中 MDA 含 量及 SOD、 CAT 和 GSH-PX 活力，酶联免疫吸附法检测 IL1β 和 TGF-β 含量． 结果表明: 与 正常对照组相比，模型组 MDA、 IL1β、 IL6 含量显著升高( P ＜ 0． 05) ， IL2 与 TGF-β 含量 显著降低( P ＜0． 05) ， SOD、 CAT、 GSH-PX 活力均显著下降( P ＜0． 05);与模型组相比， 4 种 肽均能调节皮肤组织氧化和炎症状态，且总体呈剂量依赖性关系，但不同肽之间效果差异 较大，以样品2 中剂量组效果最佳，能显著提高光老化 SD 大鼠皮肤中抗氧化酶活性，降 低脂质过氧化，改善免疫抑制和细胞微环境，可作为拮抗皮肤光老化的食品基料． 

 To investigate the regulation of four kinds of food-derived peptides with oral administration on the oxidative and inflammatory system of photoaging skin in SD rats，SD rats with SPF grade were equally distributed as normal group，model group with ultraviolet irradiation and peptides oral administration groups before UV exposure． Before experiment，about 5 cm2 of dorsal hair in all animals were completely removed off and except the animals in normal group， the other animals were exposed to UV irradiation to establish the photoaging model． At the same time， the low，middle and high-dose peptides solution was prepared by dissolving the peptides into water to obtain the concentration of 0. 3，0. 9，and 1. 5g/L， respectively． Consequently，as the sole drinking source， the peptides solution was orally administrated to the SD rats in peptides treating groups． The animals in UV and peptides administration groups were continuously exposed to UV irradiation until the photoaged model was established． After scarification，about 1g of dorsal skin was homogenated in a refiner，with 10% homogenate solution for biochemical detection． The content of methane dicarboxylic aldehyde ( MDA) and the antioxidase activity of SOD，CAT and GSHPX were detected by biochemical assay and the content of inflammatory cytokines of interleukin1β ( IL1β) ， IL2， IL6，IL10 and transforming growth factor β ( TGF-β) were determined by enzymelinked immunosorbent assay ( ELISA) ． The results illustrated that the concentration of MDA， IL1β and IL6 in skin tissue of model group were significant higher while the content of antiinflammatory cytokines of IL2， IL6， IL10 and TGF-β were significantly decreased and the anti-oxidase activity of SOD，CAT and GSH-PX were significantly lower than those in normal control group ( P ＜ 0. 05) ． In comparison with model group，peptides groups exhibited desirable and remarkable regulation on the oxidative and inflammatory status with a dose-dependency manner． On the whole， the remarkable difference among the four kinds of food-derived peptides in protecting skin from photoaging process was observed， in which the sample 2 at high dose enhanced the anti-oxidase activity， reduced the lipid peroxidation level，and improved the immunity suppression and cellular microenvironment． Therefore the peptide of sample 2 can be used as the primary ingredient for developing functional food against photoaging．