有机氯溶剂作为重要的化工原料在使用过程中可能会造成严重的环境污染． 目 前在多个有机氯污染场地地下水中均探测到顺 1，2-二氯乙烯(cDCE)浓度超标，其毒性 远大于三氯乙烯和四氯乙烯． 针对有机氯污染场地地下水中 cDCE 的微生物好氧矿化的 问题，本研究选择了某地区有机氯污染地下水作为菌源，经过338d 富集驯化，最后培养得 到了 cDCE 好氧矿化菌群． 培养得到的 cDCE 好氧矿化菌群表现出较好的代谢 cDCE 的能 力，在 cDCE 浓度为1mg/L 的初始浓度下，16d 后降解率为38． 1%． 为丰富其生物学信息， 同时针对驯化期间不同时期的菌群运用 16S rＲNA 高通量宏基因组测序技术对其进行了 分析，得到了菌群的分子生物学信息:不同培养阶段的样品有一定的连续性;其多样性随 着微生物的培养时间先降低后升高;同时得到了不同阶段微生物的群落结构，结合其他生 物学信息以及培养过程中微生物对 cDCE 的代谢能力，最终推测 cDCE 好氧矿化菌群属 于 Delftia 属．

Organic chlorine solvent，as an important chemical raw material，may lead to serious pollution in the process of use． At present，the concentration of cis 1，2-dichloroethylene (cDCE) has been detected in ground- water of many organic chlorine contaminated sites，and its toxicity is much greater than that of trichloroethylene and tetrachloroethylene． In view of the problem of microbial aerobic mineralization of cDCE in groundwater contamina- ted by organochlorine，organic chlorine contaminated groundwater was taken as a source of bacteria in a certain area． After 338 days' enrichment and domestication，the cDCE mineralized aerobic bacteria was finally cultured． The cultured cDCE aerobic mineralized mixed bacteria showed better ability to metabolize cDCE． At the initial concen- tration of cDCE concentration of 1 mg/L，the degradation rate was 38. 1% after 16 days． In order to enrich its bio- logical information，16S rRNA high-throughput metagenomic sequencing technology was used to analyze mixed bac- teria in different periods during domestication，and molecular biological information of mixed bacteria was ob- tained． The samples in different culture stages have certain continuity; their diversity decreases first and then in- crease with the culture of microorganisms． At the same time，the community structure of microorganisms at diffe- rent stages was obtained． Combined with other biological information and the ability of microorganisms to metabo- lize cDCE during culture，it is speculated that cDCE mineralized aerobic bacteria belongs to the genus Delftia．