用非序列比对方法研究微生物菌群的分类是目前生物信息学中的一个热门领域． 文中开发了一种基于 k-mer 统计的非序列比对软件 SeqDistK． SeqDistK 可通过开源网站 https: ∥github． com /htczero /SeqDistK 获得，具有在微生物菌群分类中运算速度快、准确
度高的优点，而且具有适应大型数据研究的潜力． 利用 SeqDistK 对 63 条已知分类的 16S rRNA 基因序列所算出的距离矩阵进行菌群聚类，发现所得聚类结果与已有的分类基本一致． SeqDistK 能准确地对微生物菌群样本序列进行聚类，可作为一个有效的从分子生物学角度分析系统发育学的软件．

Using sequence alignment-free comparison method to study microbial flora classification is a hot topic in bioinformatics． In this paper，SeqDistK，the sequence alignment-free comparison software based on k-mer statis- tics，is presented． The open source software package can be obtained from https: ∥github． com /htczero /SeqDistK． SeqDistK has the advantages of fast calculation and high accuracy in microbial flora classification，and has the po- tential to adapt to large-scale data research． By adopting SeqDistK to cluster 63 distance matrices of 16S rＲNA gene sequences，it is found that the clustering results are basically consistent with the existing classifications，which means that SeqDistK can accurately classify microbial flora clustering samples and provides effective software for phylogenetic analysis in molecular biology．