在含深度共熔溶剂的非水相体系中,利用固定化Kurthia gibsonii SC0312细胞高效催化外消旋1-苯基-1,2-乙二醇(PED)不对称氧化以制备对映体纯的(S)-PED.在所考察的4种离子液体和2种深度共熔溶剂中,氯化胆碱/尿素([ChCl][U])对Kurthia gibsonii SC0312细胞表现出良好的生物相容性,能适当改变细胞膜的完整性,有效提高反应的催化效率.在[ChCl][U]体积分数为4%、缓冲液p H值为7. 5、反应温度为35℃、细胞质量浓度为60mg/m L、底物浓度为100mmol/L的条件下,反应初速度为8. 25mmol/(L·h),反应仅7 h产率及(S)-PED e. e.值即分别达49. 6%和99. 9%.

Highly efficient asymmetric oxidation of racemic 1-phenyl-1，2-ethanediol ( PED) was successfully con- ducted to prepare enantiomerically pure ( S) -PED with immobilized Kurthia gibsonii SC0312 cells in non-aqueous system containing DESs． Among the 4 kind of tested ionic liquids and 2 kind of deep eutectic solvents，［ChCl］［U］ manifested better biocompatibility with Kurthia gibsonii SC0312 cells，and it can appropriately change the cell membrane integrity，thereby effectively improve the catalytic efficiency of this reaction． When ［ChCl］［U］content is 4% ( volume fraction) ，buffer pH is 7． 5，reaction temperature is 35 ℃，cell concentration is 60 mg /mL，and substrate concentration is 100 mmol /L，the initial reaction rate reaches 8． 25 mmol /( L·h) ，and after 7 hours' re- action，the yield rate and ( S) -PED e． e． value reach 49． 6% and 99． 9% ，respectively．