食品科学与技术

植物乳杆菌发酵大豆分离蛋白凝胶的特性分析

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  • 东北农业大学 食品学院,黑龙江 哈尔滨 150030
李良( 1981-) ,男,博士,副教授,主要从事粮食油脂及植物蛋白工程研究

收稿日期: 2018-09-25

  修回日期: 2018-11-16

  网络出版日期: 2019-01-31

基金资助

国家重点研发计划项目( 2018YFD0400500,2018YFD0400503) ; 黑龙江省博士后项目( LBH-Z15021) ; 东北农业 大学学术骨干项目( 18XG28 )

Analysis on the Characteristics of Soybean Protein Isolate Gel Fermented by Lactobacillus plantarum

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  •  College of Food Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
李良( 1981-) ,男,博士,副教授,主要从事粮食油脂及植物蛋白工程研究

Received date: 2018-09-25

  Revised date: 2018-11-16

  Online published: 2019-01-31

Supported by

Supported by the National Key Research and Development Program ( 2018YFD0400500,2018YFD0400503) and the Post-Doctoral Project of Heilongjiang Provience ( LBH-Z15021)

摘要

本研究以大豆分离蛋白( SPI) 为原料,通过植物乳杆菌发酵制备蛋白凝胶,并对 凝胶工艺和聚集体的性质进行分析. 正交试验结果表明: SPI 凝胶最优接种量为 3% ,发酵 时间为 5 h,发酵温度为 37 ℃,初始 pH 值为 6. 5. 此条件下持水性为 46. 00% ,凝胶强度为 23. 67 g. 研究发现,植物乳杆菌发酵对蛋白浊度、电位和平均粒径均产生了显著性影响. 实 验结果有助于阐明乳酸菌发酵处理对豆类蛋白凝胶的影响,从而进一步为益生菌植物乳 杆菌在豆制品中的应用提供理论指导.

本文引用格式

李良 杨晓宇 王雁 苏悦 江连洲 . 植物乳杆菌发酵大豆分离蛋白凝胶的特性分析[J]. 华南理工大学学报(自然科学版), 2019 , 47(3) : 93 -100 . DOI: 10.12141/j.issn.1000-565X.180477

Abstract

Soybean protein isolate ( SPI) as the raw material,protein gels were prepared by Lactobacillus plantarum fermentation. Both the gel process and the properties of the aggregation were analyzed. The experimental results show that: the optimal quantity of Lactobacillus plantarum is 3% ,the fermentation time is 5 h,and fermentation temperature is 37 ℃,initial pH value is 6. 5. Under this conditions,the water holding capacity of the gel is 46. 00% ,and the gel strength is 23. 67g. It finds that the Lactobacillus plantarum fermentation has a significant influence on protein turbidity,Zeta-potential and the average particle size. The experimental results are useful for clarifying the influence of Lactobacillus fermentation treatment on soy protein gel,and further provide a theoretical guidance for the application of probiotic Lactobacillus in soy products.

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