高黏度液体的pL 级微量分配和狭小空间内的点样是微量液体分配的难点，针对这个问题，文中从机理上分析了影响转印式pL 级点样性能的因素，通过实验研究了液体
黏度、移液针的移动速度、停留时间、液窝对点样性能的影响，实现了高黏度( 5、30 Pa·s)的pL 级液体分配和窄缝内( 0. 5mm × 20mm) 的微量点样． 研究结果表明: 低速点样时，液体分配量随着液体黏度的增加呈增加趋势，且受移液针停留时间的影响较小; 高速点样时，液体黏度对液体分配量的影响较小，但移液针的停留时间对点样量有较大的影响; 通过合理匹配参数，转印式液滴分配方法可以有效地抑制液窝的生成，减小随机因素的影响，实现高黏度液体的高准确性、高一致性pL 级超微量点样和狭小空间内的封装点样．

In order to overcome the difficulty in the picoliter ( pL ) -class trace distribution and the dispensing in narrow spaces of high-viscosity fluid,the factors affecting the pL-class dispensing performance of transfer printing were theoretically analyzed,and some experiments were carried out to explore the influences of fluid viscosity,dispensing velocity,liquid transfer needle s holding time and fluid concave on dispensing performance.Finally,pLclass dispensing of high-viscosity ( 5Pa·s and 30Pa·s) fluid in narrow seam ( 0. 5mm × 20mm) was implemented.The results show that,at low dispensing velocity,the dispensing volume increases as fluid viscosity increases but keeps almost unchanged with the holding time of liquid transfer needle,while the contrary occurs at high dispensing velocity; and that the transfer printing liquid distribution method can effectively inhibit the formation of fluid concave and reduce random factors-induced impact with well-matched dispensing parameters.Thus,the pL-class dispensing and packing in a narrow seam of high-viscosity fluid can be successfully implemented with high accuracy and good repetitiveness.