收稿日期: 2016-05-04
修回日期: 2016-08-29
网络出版日期: 2017-02-02
基金资助
广东省自然科学基金博士启动基金资助项目( 2015A030310494) ; 广东“特支计划”科技创新青年拔尖人才项目( 2014TQ01N538)
Investigation into Identification,Antioxidant Activity and Anti-Hemolysis Activity of Phenolics Compounds in High Acid Apple
Received date: 2016-05-04
Revised date: 2016-08-29
Online published: 2017-02-02
Supported by
Supported by the Dr. Start-up Fund Projects of the Natural Science Foundation of Guangdong Province ( 2015A030310494) and the Special Support Project of Guangdong Province for Science and Technology Innovative Young Talents ( 2014TQ01N538 )
孙为正 杨坤 林恋竹 邹颖 赵谋明 . 高酸苹果多酚鉴定及抗氧化、抗溶血活性研究[J]. 华南理工大学学报(自然科学版), 2017 , 45(3) : 146 -152 . DOI: 10.3969/j.issn.1000-565X.2017.03.021
The composition of a kind of high acid apple from Xinjiang,China,namely Hongxun Ⅰ ( red flesh) ,was determined,finding that the high acid apple is of a titratable acidity of 1. 31%,a total phenolics and anthocyanins content in an order of peel > pomace > whole fruit ( with a total phenolics content of ( 2026. 88 ±28. 30) mg /kg and a total anthocyanins content of ( 244. 92 ± 13. 88) mg /kg) > flesh.Then,ten phenolic compounds including chlorogenic acid,phlorizin,epicatechin,ferulic acid,myricetin,catechins,vanillic acid,p-coumaric acid,rutin and caffeic acid were indentified by means of high-performance liquid chromatography,finding that the high acid apple is rich in chlorogenic acid ( ( 391. 19 ± 18. 37) mg /kg) ,epicatechin ( ( 698. 82 ± 17. 21) mg /kg) and catechins ( ( 570. 30 ± 9. 21) mg /kg) .Moreover,the antioxidant and anti-hemolysis activities of the high acid apple were determined by means of DPPH radical scavenging activity assay,oxygen radical absorption capacity assay and AAPH-induced oxidative damage human erythrocytes model,finding that the peel,flesh,pomace and whole fruit of the high acid apple all exhibit excellent antioxidant activity and that the anti-hemolysis activities are indicative of the following order: peel > pomace > whole fruit > flesh.All these above-mentioned statements indicate that,as a valuable product,high acid apple is worth processing and developing.
Key words: high acid apple; antioxidant activity; anti-hemolysis activity; phenolics
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