通过干热法和大分子拥挤体系分别制备大豆 7S 球蛋白 － 葡聚糖共价复合物，讨论不同反应体系制备的产物的物化性质差异． 根据糖基化产物在 7S 等电点(pH =4． 8)和中性条件(pH =6． 5)下的溶解性将其分成两组分，对其接枝度、蛋白质二级结构、内置荧
光和浊度进行分析． 结果表明:在大分子拥挤环境下，7S 在液相环境中发生了一定程度的水解，生成小分子的肽链，有利于多糖的共价接入;MC48(pH =4． 8 时制得，大分子拥挤体系)中主要是接枝程度较高的糖基化产物，MC65(pH = 6． 5 时制得，大分子拥挤体系)中含有的糖基化产物接枝度较低，且程度不均匀;干热法制备得到的 DH48(pH = 4． 8 时制得)和 DH65(pH = 6． 5 时制得) 接枝度较低，差异性不及大分子拥挤环境显著． SDS-PAGE、圆二色光谱、内置荧光分析结果表明，大分子拥挤环境下制得的 MC48 和 MC65 物化性质差异显著，而干热法制得的 DH48 和 DH65 的物化性质差异不明显．

Two kinds of β-conglycinin-dextran conjugates were prepared respectively in the macromolecular crowding environment or via the dry-heating method，and then a comparison was made in their physicochemical property． On the basis of the solubility of glycation products at the isoelectric point of β-conglycinin (pH =4. 8) or under the neutral condition (pH =6. 5)，the products were separated into two components，and then a discussion was conducted about the graft degree，secondary structure，internal fluorescence and turbidity of the two components．The results show that (1) β-conglycinin in the macromolecular crowding and liquid phase environment is hydrolyzed to a certain degree，with small peptide chains being produced，which is beneficial to the covalent access of polysaccharide; (2) MC48 obtained at pH4. 8 in the macromolecular crowding environment，is mainly the glycation product of high graft degree，while MC65 obtained at pH6. 5 in the macromolecular crowding environment，contains the glycation products of both low and uneven graft degree; and (3) DH48 and DH65 obtained respectively at pH4. 8 and pH6. 5 via the dry-heating method，are of low graft degree，and their difference is less significant than that in the macromolecular crowding environment． Moreover，it is found from SDA-PAGE，CD and intrinsic fluorescence emission spectra that there exists a significant difference in physicochemical property between MC48 and MC65，while an insignificant difference between DH48 and DH65．