So far as the cytoplasm segmentation in the process of cell microinjection is concerned,the traditionallevel set models can not directly segment cytoplasm from multiple adhered targets.Additionally,they are slow insegmentation.In order to solve these problems,a new cell segmentation method based on variation level set is pro-posed.In this method,the joint contour of cell and injection pipette is acquired according to the difference inshape.Then,by combining the variation level set theory,an extra edge constraint is added to improve the energyfunctional model.A complete cell segmentation is thus achieved and the convergence speed of the variation level setis significantly improved.Experimental results indicate that the proposed method helps to perform the independentcytoplasm segmentation rapidly and at the same time avoid wrong segmentation of other targets.