为探讨合成气发酵乙醇的新工艺，对梭菌C．autoethanogenum 的生长特性及CO 发酵性能进行了研究．考察了不同碳源对C．autoethanogenum 生长的影响，发现木糖是其生长的最佳底物．对生长培养基进行了改良，使C．autoethanogenum 的菌体质量浓度提高2 倍以上，代谢产物以乙酸为主，只产生少量乙醇．利用1L 气体采样袋研究了C．autoetha- nogenum 的CO 发酵性能，在1．0g/L 酵母膏的发酵培养基中经过两次CO 发酵后，乙醇质量浓度达3．464g/L，CO 乙醇转化率达51．7%．研究表明:N 2 环境、180℃、4MPa 下，20min 高温液态水处理蔗渣得到的水解液可用于C．autoethanogenum 的培养，培养液再进行CO 发酵，经过一次发酵后得到的乙醇质量浓度为3．13g/L，CO 乙醇转化率为46．9%，与之前两次发酵的结果接近，但是发酵时间大大缩短．

In order to develop the new technology of ethanol production from synthesis gas fermentation,the growth characteristics and carbon monoxide (CO) fermentation performance of C.autoethanogenum were investigated.The effects of different carbon sources on the growth of C.autoethanogenum were analyzed and the results show that xy- lose is the optimal substrate.After the growth medium being modified,the cell density of C.autoethanogenum in- creased by more than 2 times,and the metabolic products were mostly acetic acid with a small amount of ethanol.Moreover,the CO fermentation performance of C.autoethanogenum was studied in 1L gas sampling bags.After twice CO fermentation with C.autoethanogenum in the fermentation medium containing 1.0g/L yeast extract,an ethanol concentration of 3.464g/L was achieved,and the yield of ethanol from CO reached 51.7%.The investigation re- sults also indicate that the hydrolysate of bagasse treated with hot liquid water at 180℃ in a 4MPa N 2 atmosphere for 20min can be utilized to culture C.autoethanogenum,and the corresponding ethanol concentration and ethanol yield from CO can reach 3.13g/L and 46.9%,respectively after only once CO fermentation,which are similar to the results of the twice CO fermentation but with much less culture time.