收稿日期: 2013-08-21
修回日期: 2013-09-30
网络出版日期: 2013-11-19
基金资助
国家国际科技合作专项(2013DFA41670);国家自然科学基金资助项目(21376100);国家杰出青年科学基金资助项目(20925622)
Effect of pH Value on Cellulase Adsorption on Wheat Straw Alkaline Lignin
Received date: 2013-08-21
Revised date: 2013-09-30
Online published: 2013-11-19
Supported by
国家国际科技合作专项(2013DFA41670);国家自然科学基金资助项目(21376100);国家杰出青年科学基金资助项目(20925622)
楼宏铭 李秀丽 王梦霞 邱学青 杨东杰 邓永红 . pH 值对纤维素酶在麦草碱木质素上吸附的影响[J]. 华南理工大学学报(自然科学版), 2013 , 41(12) : 1 -5 . DOI: 10.3969/j.issn.1000-565X.2013.12.001
Quartz crystal microbalance was used to detect the adsorption capacity and configuration variation of cel-lulase on wheat straw alkaline lignin film,and the effect of pH value on the adsorption was investigated.The resultsindicate that (1) there exists electrostatic repulsion between cellulase and wheat straw alkaline lignin when the pHvalue ranges from 4.8 to 6.0; (2) the electrostatic repulsion becomes stronger and the cellulase adsorption on thelignin film becomes more unfirm at a higher pH value,thus loosening the adsorbed film and decreasing the adsorp-tion capacity; and (3) high pH value benefits the desorption of cellulase from wheat straw alkaline lignin film andits recovery.
Key words: cellulase; lignin; adsorption; desorption; quartz crystal microbalance
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