以丙酮丁醇梭菌为发酵用菌株发酵水蒸气爆破玉米秸秆酶解上清液生产丁醇，研究了酶解上清液浓度、初始 pH 值以及菌液接种量对丁醇产量的影响，并通过正交试验来确定营养元素的最佳添加量．结果表明: 在丙酮丁醇梭菌发酵水蒸气爆破玉米秸秆酶解上清液生产丁醇的最佳条件下( 上清液糖浓度 57．5g/L、初始 pH 值6. 3，菌液接种量6%，发酵温度 37℃， 营养元素酵母膏、乙酸铵、磷酸二氢钾、烟酰胺添加量分别为 0. 8、6. 0、0. 5、0. 25g/L) ， 丁醇产量达9. 726g/L; 水蒸气爆破玉米秸秆酶解液中有抑制产生溶剂的物质，少量烟酰胺能有效促进丁醇生产．

In this paper,butanol was prepared from the enzymatic hydrolysate supermatant of steam- exploded cornstover,with Clostridium acetobutylicum as the experimental bacterium. Then,the effects of supermatant concentra-tion,initial pH value and microbial inoculation quantity on butanol yield were investigated,and the optimal dosagesof nutrient elements were determined by orthogonal test.The results show that,under optimal conditions,that is,asupermatant sugar content of 57.5g/L,an initial pH value of 6.3,an inoculation quantity of 6%,a fermentationtemperature of 37 ℃,and a nutrient elements addition of yeast extract 0.8g/L + CH₃ COONH₄ 6. 0g/L + KH₂ PO₄ 0. 5g/L + nicotinamide 0.25g/L,the butanol yield is up to 9.726g/L.Moreover,it is also found that there existsome inhibitors for the solvent production from steam- exploded corn stover enzymatic hydrolysate,and that a smallamount of nicotinamide may effectively promote butanol production.