收稿日期: 2013-06-03
修回日期: 2013-07-23
网络出版日期: 2013-09-03
基金资助
国家自然科学基金重点资助项目( 31130042) ; 广东省科技计划项目( 2012A020602005)
Hydrolysis Characteristics of β-Amylase on Starches from Different Sources
Received date: 2013-06-03
Revised date: 2013-07-23
Online published: 2013-09-03
Supported by
国家自然科学基金重点资助项目( 31130042) ; 广东省科技计划项目( 2012A020602005)
黄强 陈磊 扶雄 . β-淀粉酶对不同品种淀粉的水解特性[J]. 华南理工大学学报(自然科学版), 2013 , 41(10) : 60 -65 . DOI: 10.3969/j.issn.1000-565X.2013.10.010
The waxy cornstarch,the normal cornstarch,the potato starch and the tapioca starch were gelatinizedand were then hydrolyzed by using β-amylase,and the hydrolyzates were investigated in terms of the fluidity,thedextrose equivalent ( DE) value,the relative molecular mass distribution,and the thermodynamic property. The resultsshow that ( 1) at the β-amylase dosage of 0.1%,the waxy corn-starch possesses the largest fluidity,while thenormal corn starch possesses the lowest fluidity; ( 2) at the β-amylase dosage of 0.3%,the fluidity of the hydrolyzatesafter a 5 h reaction become stable; ( 3) when the β-amylase dosage is more than 0.3%,the DE values of thehydrolyzates from cereal starches ( i.e. the waxy corn-starch and the normal corn-starch) is greater than those oftube starches( i.e. the potato starch and the tapioca starch) ; ( 4) the relative molecular masses of the hydrolyzatesfrom cereal starches exhibit a narrow distribution,while the relative molecular masses of the hydrolyzates of tubestarches are significantly different and are higher than those of cereal starches; and ( 5) the hydrolyzates of the normalcorn starch show the absorption peaks of the retrograded amylose or the starch-lipid complexes,while thehydrolyzates of the other three starches exhibit no absorption peak.
Key words: β-amylase; hydrolysis; fluidity; dextrose equivalent value; thermodynamic property
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