为探讨大豆11S 球蛋白代替牛血清白蛋白( BSA) 作为酶联免疫检测的新型免疫原载体的可行性，分别采用不同方法将磺胺二甲氧嘧啶( SDM) 同大豆11S 球蛋白、大豆7S 球蛋白和BSA 交联起来作为免疫原，得到抗SDM-大豆11S 球蛋白、SDM-大豆7S 球蛋白和SDM-BSA 的多克隆抗体; 然后采用间接ELISA 法检测3 种抗血清的效价及纯化所得抗体的特异性，建立SDM 对3 种抗体的标准抑制曲线．结果表明，同SDM-BSA 相比，SDM-11S 具有较好的免疫原性和特异性，抗SDM-11S 的多克隆抗体对其他磺胺类物质的交叉反应性良好，均低于0. 01%．因此，在免疫检测中，大豆11S 球蛋白可以尝试替代牛血清白蛋白( BSA) 作为一种新型的免疫原载体．

This paper deals with the feasibility of substituting soy 11S globulin ( 11S) for bovine serum albumin ( BSA) as a neotype immunogen carrier in the enzyme-linked immunosorbent assay ( ELISA) . In this investigation,first,sulfadimethoxine ( SDM) respectively conjugated to soy 11S globulin,soy 7S globulin and BSA was used as immunogen,and the corresponding polyclonal antibodies against SDM-soy 11S globulin,SDM-soy 7S globulin and
SDM-BSA were obtained. Then,a competitive ELISA and an indirect competitive ELISA were conducted to detect the titers of the three antiserums and the specificity of the purified antibodies. Finally,representative standard curves of SDM inhibiting the three antibodies were presented. The results indicate that,as compared with SDMBSA,SDM-11S conjugate is of better immunogenicity and specificity,and that the cross reactivities of SDM-11S
antibody against other sulfonamides are all excellent,with a value being less than 0. 01%. All these results verify the feasibility of substituting soy 11S globulin for BSA as a neotype immunogen carrier in ELISA.