雷帕霉素靶蛋白( TOR) 在调节动植物的生长及新陈代谢方面起重要作用．为进一步研究植物中TOR 的作用，对盐芥TOR 基因进行克隆，采用聚合酶链式反应和cDNA末端快速扩增技术等分子生物学方法获得盐芥TOR 基因的cDNA 全长及基因组全长，构建了ThTOR 基因沉默植物表达载体并成功获得盐芥ThTOR 基因沉默的转基因株系．结果表明: ThTOR cDNA 全长为7 836 bp，编码2 479 个氨基酸，蛋白序列与拟南芥同源性高达97. 5%，基因组全长19162 bp; 盐芥ThTOR 基因沉默的转基因株系具有生长缓慢、花及种子的发育受阻的明显表型．

Mammalian target of rapamycin ( TOR) plays an important role in the growth regulation and metabolism of animals and plants. In order to reveal the function of TOR in plants,TOR gene of Thellungiella halophila ( T. halophila) was cloned,and the full lengths of ThTOR cDNA and genomic DNA were obtained by polymerase chain reaction ( PCR) and rapid amplification of cDNA ends ( RACE) . Then,RNAi vector ThTOR-RNAi was constructed
and the transgenic lines were obtained by transforming ThTOR-RNAi into T, halophila. The results show that the full-length ThTOR cDNA contains 7836 base pairs and encodes a protein with 2479 amino acids,that a high degree of homology ( 97. 5%) exists between the proteins encoded by ThTOR and AtTOR,that the full-length ThTOR genomic DNA is of 19 162 base pairs,and that obvious phenotypes of slow growth as well as flowers and seeds abortion occur in T. halophila ThTOR-RNAi transgenic lines.