华南理工大学学报(自然科学版) ›› 2005, Vol. 33 ›› Issue (7): 42-46.

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好氧反硝化菌株X31的反硝化特性

马放 王弘宇 周丹丹 左微   

  1. 哈尔滨工业大学 市政环境工程学院,黑龙江 哈尔滨 150090
  • 收稿日期:2004-11-05 出版日期:2005-07-25 发布日期:2005-07-25
  • 通信作者: 马放(1963-),男,教授,博士生导师,主要从事环境生物技术和水污染控制的研究 E-mail:whydream2000@ eyou.com
  • 作者简介:马放(1963-),男,教授,博士生导师,主要从事环境生物技术和水污染控制的研究
  • 基金资助:

    国家自然科学基金重大国际合作项目(50521140075)

Denitrification Characteristics of an Aerobic Denitrifying Bacterium Pseudomonas C loritidismutans Strain X31

Ma Fang  Wang Hong-yu  Zhou Dan-dan  Zuo Wei   

  1. School of Municipal and Environmental Engineering,Harbin Institute of Tech.,Harbin 150090,Heilongjiang,China
  • Received:2004-11-05 Online:2005-07-25 Published:2005-07-25
  • Contact: 马放(1963-),男,教授,博士生导师,主要从事环境生物技术和水污染控制的研究 E-mail:whydream2000@ eyou.com
  • About author:马放(1963-),男,教授,博士生导师,主要从事环境生物技术和水污染控制的研究
  • Supported by:

    国家自然科学基金重大国际合作项目(50521140075)

摘要: 对好氧反硝化菌株X31在好氧条件下的反硝化特性进行了研究.结果表明,反硝化主要发生在茵体的对数生长期,氮气是反硝化过程的最终产物.在反硝化过程中,pH值呈逐渐上升趋势,而氧化还原电位(ORP)呈逐渐降低趋势.菌株X31能够以硝酸盐或亚硝酸盐和氧气为电子受体进行协同呼吸,并且亚硝酸盐呼吸要较硝酸盐呼吸更容易进行.硝酸盐呼吸和亚硝酸盐呼吸都具有较高的脱氮效率.和其他已报道的好氧反硝化茵相比,X31菌株有着更高的氧耐受浓度.当培养液中初始的氧化态氮质量浓度为150mg/L左右时,溶解氧值对X31菌株的反硝化效果没有显著的影响.

关键词: 好氧反硝化, 硝酸盐呼吸, 亚硝酸盐呼吸, 生物脱氮, 协同呼吸, 电子受体

Abstract:

The denitrification characteristics of an aerobic denitrifier,Pseudomonas chloritidismutans strain X31,Were studied under aerobic conditions.The results show that:(1)the dentitrification process of X31 mainly occurs in the LOG phase of the strain and the major end product of the denitrification is molecular nitrogen,(2)during the denitrification of X31,the pH in the culture medium gradually increases,while the Oxidation-reduction Poten-tial gradually decreases,(3)X31 can co-respire by using nitrate or nitrite and oxygen as the electron acceptor,and (4)nitrite respiration is easier than nitrate respiration,both of which achieve high nitrogen removal rate.It is also shown that X31 possesses a higher tolerance to dissolved oxygen than other aerobic denitrifiers reposed previously,and that,when the initial mass concentration of nitrogen oxides in the cuhure medium is about 1 50 mg/L,the aero-bic denitrification rate of the strain is not obviously affected by dissolved oxygen.

Key words: aerobic denitrification, nitrate respiration, nitrite respiration, biological nitrogen removal, co-respira-tion, electron acceptor